Ac-NLWAAQRYGRELRRMSDK(fluorescein)FVD-OH Yello lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. A fluorescein-labeled Bad BH3 peptide that serves as an useful probe for investigating the nature of Bcl-xL- peptide interaction and as a substrate for high-throughput screening of Bcl-xL inhibitors based on fluorescence polarization. Interacts strongly with Bcl-xL with a Kd of 21.48 nM. The assay tolerates up to 8% DMSO. Purity: ≥95% by HPLC. Excitation max.: ~485 nm, Emission max.: ~530 nm Ac-Asn-Leu-Trp-Ala-Ala-Gln-Arg-Tyr-Gly-Arg-Glu-Leu-Arg-Arg-Met-Ser-Asp-Lys(FITC-I)-Phe-Val-Asp-OH Ref.: Zhang, H., et al. 2002. Anal. Biochem. 307, 70 |