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 | 2 to 8°C |  | Blue ice |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Innolink™ Biotin 354S Kit | |  |
|  | Cat. No. 203120 |
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| Format: Protein conjugation | | Detection Method: UV spectrophotometry | | Kit Contents: Biotin 345S, DMF Anhydrous, 10X Modification Buffer, Diafiltration Apparatus, and a user protocol. | | Comments: Innolink™ Biotin 354 S, incorporates a UV-traceable biotin into any primary amine-containing biomolecule or surface and allows for direct spectrophotometric quantification of total biotin. Innolink™ Biotin 354S is designed to (a) incorporate a bis-aryl hydrazone chromophore (e = 29,000 @ 354 nm) within the biotin linker arm that allows for direct spectroscopic quantification of total incorporated biotins, (b) a long chain PEG4 linker to preserve biotin/avidin affinity as well as increase solubility and (c) an aromatic succinimidyl ester that more efficiently modifies amines in aqueous buffers than aliphatic succinimidyl esters. | | Ref.: Steinberg-Tatman, G., et al. 2006. Bioconjug. Chem. 17, 841. Kline, M.C., et al. 2005. J. Forensic Sci. 50, 1. Chen, Y., et al. 2004. Chem. Commun. 24, 2804. Kozlov, I.A., et al. 2004. Biopolymers 73, 621. Zhong, X.B., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 11559. Hartmann, D.M., et al. 2002. Journal of Materials Research 17, 473. | | R: 10-20/21-36-61; S: 36/37-45-53 |
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Spectra of Desalted Samples at Various Biotin:Protein Mole Equivalents. Superimposed spectra of desalted Innolink™ biotin reactions at various biotin:protein mole equivalents.
Streptavidin Binding Efficiency. Biotinylated bovine IgG was treated with 1 and 2 mole equivalents of streptavidin and binding was confirmed by PAGE gel-shift analysis. The data indicates that there is increasing streptavidin binding efficiency with increasing biotin modification of bovine IgG. The lack of complete streptavidin binding (gel-shift) at the 5X modification level is likely due to the inaccessibility of some biotins as they may be 'hiding' in hydrophobic pockets
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