Liquid. Supplied at 10 mg/ml in 20 mM HEPES, pH 7.5. AVOID FREEZE/THAW CYCLES. Nucleoside antibiotic that specifically inhibits protein synthesis in both prokaryotes and eukaryotes. Suitable for selection of cells carrying plasmids conferring blasticidin resistance. Blasticidin resistance is conferred by the blasticidin S deaminase gene (bsr) that converts blastisidin S to a nontoxic deaminohydroxy derivative. CAS 3513-03-9. Merck Index: 14, 1316 Ref.: Kimura, M., et al. 1994. Biochim. Biophys. Acta 1219, 653. Kimura, M., et al. 1994. Mol. Gen. Genet. 242, 121. Kojima, N., et al. 1994. J. Biol. Chem. 269, 30451. Kudo, T., et al. 1994. J. Immunol. Methods 177, 17. Sutoh, K. 1993. Plasmid 30, 150. Izumi, M., et al. 1991. Exp. Cell Res. 197, 229. Yamaguchi, H., et al. 1965. J. Biol. Chem. 57, 667. Takeuchi, S., et al. 1958. J. Antibiot. 11, 1. |