Bone Morphogenetic Protein-6 Lyophilized. Lyophilized from a 0.2 µm filtered solution of 35% ACN, 0.1% TFA, 50 µg BSA/µg BMP-6. Recombinant, mouse bone morphogenetic protein-6 expressed in NSO cells. An osteogenic protein belonging to the transforming growth factor-b (TGF-b) superfamily. BMP-6 has been shown to strongly induce phosphorylation and nuclear accumulation of Smad5. BMPs have also been shown to be involved in embryogenesis and morphogenesis of cells of osteoblastic lineage. A positive regulator of keratinocyte differentiation. MW: 18000 + 23000 (reducing conditions). Biological activity: ED50 = 50-150 ng/ml based on the ability to induce alkaline phosphatase expression in MC3T3-E1 cells. Purity: ≥95% by SDS-PAGE. Contaminants: endotoxin: ≤100 pg/µg cytokine. M.W. 18,000 and 23,000 under reducing conditions. Ref.: McDonnell, M.A., et al. 2001. Exp. Cell. Res. 263, 265. Ebisawa, T., et al. 1999. J. Cell Sci. 112, 3519. Raida, M., et al. 1999. Int. J. Cancer 83, 38. Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139. Reddi, A.H. 1998. Nature Biotech. 16, 247. Honda, Y., et al. 1997. Calcif. Tissue Int. 60, 297.
R: 23/24/25-36/37/38; S: 26-36/37/39-45 |
