Bone Morphogenetic Protein Receptor-IB/Fc Chimera

Lyophilized. Lyophilized from a 0.2µm sterile-filtered PBS. Recombinant, human BMPR-IB (amino acids Lys¹⁴-Arg¹²⁶) fused at the N-terminus to a signal peptide sequence from CD33 and fused at the C-terminus to a 6X His•Tag^® Fc region of human IgG₁ via a polypeptide linker. The recombinant receptor chimera is expressed as a soluble disulfide-linked homodimer with serine/threonine kinase activity. Biological activity: ED₅₀ = 0.5-1.5 µg/ml as measured by the ability to inhibit alkaline phosphatase production induced by 200 ng/ml of recombinant human BMP-4 in C2C12 cells. Purity: ≥90% by SDS-PAGE. Contaminants: Endotoxin: ≤100 pg/µg BMP Receptor IB/Fc Chimera. M.W. 50,000 - 55,000 under reducing conditions.

Ref.: Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139. Ide, H., et al. 1997. Oncogene 14, 1377.