5b, 20(22)-Bufadienolide-3b, 14-diol White solid. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A cardiotonic steroid isolated from toads of Bufo species that potently inhibits ouabain-sensitive Na+,K+-ATPase activity (IC50 = 1.4 nM). Induces apoptosis in various human cell lines such as leukemia HL-60 and U937 cells by altering the expression of apoptosis-related genes. Reported to stimulate the activities of p21 activated kinase (PAK) and c-Jun Kinase (JNK) in leukemic cell lines. Induces a transient increase in the expression of Tiam1 mRNA in U937 cell lines (~100 nM). Bufalin also down-regulates gene expression of the intracellular signaling protein 14-3-3 in rat lens, probably a consequence of Na+,K+-ATPase inhibition. Reduces the level of topoisomerase II in human leukemia cells. Purity: ≥98% by TLC. RTECS EI2962500, CAS 465-21-4. Ref.: Kurosawa, M., et al. 2000. Am. J. Physiol. 278, C500. Kawazoe, N., et al. 1999. Oncogene 18, 2413. McGowan, M.H., et al. 1999. J. Pharmacol. Exp. Ther. 289, 1559. Watabe, M., et al. 1998. Oncogene 16, 779. Hashimoto, S., et al. 1997. Leuk. Res. 21, 875. Morris, J.F., et al. 1997. Comp. Biochem. Physiol. A Physiol. 118, 599. Brownlee, A.A., et al. 1990. Clin. Sci. 78, 169.
R: 26/27/28; S: 22-36/37/39-45 |
