CAL-AM
Calcein-acetoxymethyl Ester, Diacetate

Off-white solid. PROTECT FROM LIGHT. PACKAGED UNDER INERT GAS. A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein. Can also be used for imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis, and multidrug resistance (MDR). Purity: ≥90% by HPLC. Excitation max.: 494 nm, Emission max.: 517 nm CAS 148504-34-1.

Ref.: Dhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315. Essodaigui, M., et al. 1998. Biochemistry 37, 2243. Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177. Feller, N., et al. 1995. Br. J. Cancer 72, 543. Feller, N., et al. 1995. FEBS Lett. 368, 385.