ITSFEEAKGLDRINERMPPRRDAMP Lyophilized. Supplied as a trifluoroacetate salt. HYGROSCOPIC. Specific calcineurin inhibitor. Corresponds to the C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin (PP2B). Inhibits Mn2+-stimulated calcineurin activity (IC50 = 10 µM using 32P-myosin light chain as a substrate) but has no effect on Ni2+-stimulated enzyme activity. Does not inhibit CaM kinase II, protein phosphatase 1 or 2A. Purity: ≥97% by HPLC. Sold on the basis of peptide content. H-Ile-Thr-Ser-Phe-Glu-Glu-Ala-Lys-Gly-Leu-Asp-Arg-Ile-Asn-Glu-Arg-Met-Pro-Pro-Arg-Arg-Asp-Ala-Met-Pro-OH Ref.: Perrino, B.A., et al. 1995. J. Biol. Chem. 270, 340. Yokoyama, Y., and Wang, J.H. 1994. FEBS Lett. 337, 128. Yokoyama, N., et al. 1993. Arch. Biochem. Biophys. 300, 615. Matsui, H., et al. 1991. Biochem. Int. 24, 1119. Hashimoto, Y., et al. 1990. J. Biol. Chem. 265, 1924. |
