PP2B Assay Kit, Colorimetric

Format: 96-well plate

Form: 192 Tests

Detection Method: Colorimetric

Species Reactivity: human, mouse, rat

Assay Time: 7 h

Sample Type: Tissue/cellular extracts

Kit Contents: Human Recombinant Calcineurin as a Positive Control, Bovine Brain Calmodulin, Calcineurin Substrate (Cat. No. 207008), Assay Buffer, EGTA Buffer, Lysis Buffer, Protease Inhibitor Cocktail, Phosphate Detection Agent, Phosphate Standard, Okadaic Acid (Cat. No. 495604), Desalting Column Resin, Chromatography Column, ½ volume 96-Well Plate, and a user protocol.

Comments: A colorimetric assay kit for measuring cellular calcineurin (PP2B) enzyme activity. Assays are performed in a convenient 96-well plate format with all reagents necessary for measuring calcineurin activity. The RII Phosphopeptide Substrate (Cat. No. 207008), supplied with this kit, is the most efficient and selective substrate known for calcineurin. The free phosphate released is detected using malachite green.

Ref.: Mondragon, A., et al. 1997. Biochemistry 36, 4934. Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109. Enz, A., et al. 1994. Anal. Biochem. 216, 147. Harder, K.W., et al. 1994. Biochem. J. 298, 395. Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.

R: 21/22-36/38; S: 26-24/25