Calmodulin, Human, Recombinant Liquid. In PBS, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138). AVOID FREEZE/THAW CYCLES. Full-length, recombinant human calmodulin fused to a His•Tag® sequence at the N-terminus. Contains four functional Ca2+-binding sites (aa 20-31; aa 56-67; aa 93-104; aa 129-140) with EF-hands (aa 7-42; aa 43-78; aa 80-115; aa 116-148); and a ubiquitination site at Lys21. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Through its interaction with Ca2+, calmodulin mediates the control of a large number of enzymes, including protein kinases and phosphatases. It is expected to be phosphorylated at Thr44 by CaMK4. In addition, it serves as a substrate for various protein tyrosine kinases. Purity: >90% by SDS-PAGE. Ref.: Chou, J.J., et al. 2001.Nat. Struct. Biol.8, 990. Lennon, G., et al. 1996.Genomics33, 151. |
