CaM Kinase II Liquid. In 25 mM Tris-HCl, 10 mM DTT, 500 µM EDTA, 10 µM AEBSF, 100 µg/ml leupeptin, 50% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Native Ca2+/calmodulin kinase II from rat brain. A multi-functional calcium and calmodulin-dependent protein kinase that regulates a number of cellular functions including neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis. Reduced levels are reported following ischemia and neuronal injury. Consists of subunits of M.W. 50,000 and 58,000 - 60,000 in a 3:1 ratio. Specific activity: ≥1.0 µmol/min/mg protein. One unit is defined as the amount of enzyme that will transfer 1.0 µmol phosphate to synapsin I per min at 30°C. Purity: ≥95% by SDS-PAGE. CAS 9026-43-1. Ref.: Takeuchi, Y., et al. 2000. J. Neurochem. 74, 2557. Enslen, H., and Soderling, T.R. 1994. J. Biol. Chem. 269, 20872. Lisman, J. 1994. Trends Neurosci. 17, 406. Bading, H., et al. 1993. Science 260, 181. Machu, T.K., et al. 1993. J. Neurochem. 61, 375. Shackelford, D.A., et al. 1993. J. Neurochem. 61, 738. Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559. Browning, M.D., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 1315. Patton, B.L., et al. 1990. J. Biol. Chem. 265, 11204. Kelly, P.T., and Shenolikar, S. 1987. Methods Enzymol. 139, 690. |
