m-Calpain Liquid. In 20 mM imidazole-HCl, 5 mM b-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8. AVOID FREEZE/THAW CYCLES. Native calpain-1 from porcine erythrocytes. Ca2+-dependent heterodimeric cysteine proteinase with low Ca2+ requirement (EC50= 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin. Specific activity: ≥1200 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 9 fluorogenic units pI 5.3. EC 3.4.22.17. Ref.: Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323. Sorimachi, H., et al. 1997. Biochem. J. 328, 721. Kampfl, A., et al. 1997. J. Neurotrauma 14, 121. Johnson, G.V.W., and Gutmann, R.P. 1997. BioEssays 19, 1011. Bartus, R.T., et al. 1995. Neurol. Res. 17, 249. Wang, K.K.W., and Yuen, P.-W. 1994. Trends Pharmacol. Sci. 15, 412. Saito, K., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2628. Goll, D.E., et al. 1992. BioEssays 14, 549. Ishii, H., et al. 1992. Biochim. Biophys. Acta 1175, 37. Melloni, E., and Pontremoli, S. 1989. Trends Neurosci. 12, 438. Ross, E., and Schatz, G. 1973. Anal. Biochem. 54, 304.
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