m-Calpain Liquid. In 20 mM imidazole, 5 mM b-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8. AVOID FREEZE/THAW CYCLES. Native calpain-1 from human erythrocytes. Ca2+-dependent heterodimeric cysteine proteinase with low Ca2+ requirement (EC50= 2 µM). Specific activity: ≥1000 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 1 fluorogenic unit. Purity: Comparable to reference lot by SDS-PAGE.. Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. EC 3.4.22.17. Ref.: Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323. Sorimachi, H., et al. 1997. Biochem. J. 328, 721. Croall, D.E., and McGrody, K.S. 1994. Biochemistry 33, 13223.
R: 36/37/38-42/43; S: 26-36 |
