m-Calpain Liquid. In 20 mM imidazole-HCl, 5 mM b-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8. AVOID FREEZE/THAW CYCLES. Native calpain-2 from porcine kidney. Ca2+-dependent heterodimeric cysteine proteinase with high Ca2+ requirement (EC50 = 1 mM). The Ca2+ requirement for proteolysis of nuclear matrix proteins is dramatically decreased in the presence of DNA. Specific activity: ≥1000 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). 1 caseinolytic unit = 7 fluorogenic units. pI 4.6. EC 3.4.22.17. Ref.: Kampfl, A., et al. 1997. J. Neurotrauma 14, 121. Sorimachi, H., et al. 1997. Biochem. J. 328, 721. Mellgren, R.L., et al. 1993. J. Biol. Chem. 268, 653. Ross, E., and Schatz, G. 1973. Anal. Biochem. 54, 304.
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