H-K(FAM)-EVY~GMMK(DABCYL)-OH
m-Calpain Substrate, Fluorogenic Red lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of a-spectrin. It is not recognized by trypsin or a-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence. Purity: ≥95% by HPLC. Excitation max.: ~490 nm, Emission max.: ~518 nm H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH Ref.: Mittoo, S., et al. 2003. Anal. Biochem. 319, 234. | 
