H-E(EDANS)PLF~AERK(DABCYL)-OH Red lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Purity: ≥97% by HPLC. Excitation max.: ~505 nm, Emission max.: ~335 nm H2N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO2H Ref.: Cuerrier, D., et al. 2007. J. Biol. Chem. 282, 9600. Cuerrier, D., et al. 2005. J. Biol. Chem. 280, 40632. |
