Lyophilized. Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0. AVOID FREEZE/THAW CYCLES. Calmodulin (CaM) purified from porcine brain. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates processes like inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for applications requiring active pure calmodulin. Specific activity: ≥ 35,000 U/mg protein. One unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3'5'-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca²⁺ in a 3 ml reaction volume at 30°C, pH 7.5. Purity: >98% by SDS-PAGE.

Ref.: Massom, L., et al. 1990. Biochemistry 29, 671.