Ac-WEHD-AFC Yellow solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. A fluorogenic substrate for caspase-5 (ICErel-III, TY), caspase-1, and caspase-4. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Purity: ≥95% by HPLC. Excitation max.: ~400 nm, Emission max.: ~505 nm Ac-Trp-Glu-His-Asp-AFC Ref.: Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. |
