Ac-LEHD-AFC White solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Purity: ≥95% by HPLC. Excitation max.: ~400 nm, Emission max.: ~505 nm Ac-Leu-Glu-His-Asp-AFC Ref.: Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263. Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. |
