FLICE
MACH
Mch5

Liquid. In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human caspase-8 fused at the N- and C- termini to a His•Tag^® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 11000 and 18000. One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-IETD-pNA (Cat. No. 368057) per min at 30°C, pH 7.4. Purity: ≥90% by SDS-PAGE. M.W. 11,000 and 18,000 (heterodimer).

Ref.: Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Boldin, M.P., et al. 1996. Cell 85, 803. Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464. Muzio, M., et al. 1996. Cell 85, 817.