FLICE/MACH/Mch5 Assay Kit | Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric or Fluorimetric | | Assay Time: 2.5-3.5 h | | Sample Type: Purified caspase-8 | | Kit Contents: Human Recombinant Caspase-8 (Cat. No. 218769), an IETD-pNA Colorimetric Substrate, Calibration Standard, Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol. | | Comments: A convenient assay kit useful for measuring the protease activity of caspase-8. Cleavage is monitored colorimetrically by an increase in absorbance at 405 nm due to the release of pNA. Useful for screening caspase-8 inhibitors. | | Ref.: Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Medema, J.P., et al. 1997. EMBO J. 16, 2794. Muzio, M., et al. 1997. J. Biol. Chem. 272, 2952. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578. Boldin, M.P., et al. 1996. Cell 85, 803. Muzio, M., et al. 1996. Cell 85, 817. Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464. Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486. Chinnaiyan, A.M., et al. 1995. Cell 81, 505. | |