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Kit Resource
  Caspase-8 Assay Kit
Cat. No. 218770  
All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase

FLICE/MACH/Mch5 Assay Kit

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric or Fluorimetric
Assay Time: 2.5-3.5 h
Sample Type: Purified caspase-8
Kit Contents: Human Recombinant Caspase-8 (Cat. No. 218769), an IETD-pNA Colorimetric Substrate, Calibration Standard, Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol.
Comments: A convenient assay kit useful for measuring the protease activity of caspase-8. Cleavage is monitored colorimetrically by an increase in absorbance at 405 nm due to the release of pNA. Useful for screening caspase-8 inhibitors.
Ref.: Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Medema, J.P., et al. 1997. EMBO J. 16, 2794. Muzio, M., et al. 1997. J. Biol. Chem. 272, 2952. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578. Boldin, M.P., et al. 1996. Cell 85, 803. Muzio, M., et al. 1996. Cell 85, 817. Fernandes-Alnemri, T., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 7464. Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486. Chinnaiyan, A.M., et al. 1995. Cell 81, 505.

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Related Literature:

Caspases and other Apoptosis Related Tools Brochure

Antibodies for Cancer Research Product Guide

Tools and Tips for Analyzing Apoptosis

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218770: Caspase-8 Assay Kit - English
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