ICE
IL-1b Converting Enzyme Liquid. In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000. Activity: ≥50,000 units/ml. Specific activity: ≥25,000 units/mg protein. One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4. Purity: ≥90% by SDS-PAGE. Ref.: Thornberry, N.A. 1994. Methods Enzymol. 244, 615. Thornberry, N.A., et al. 1992. Nature 356, 768. |
