FLICE-2
Mch4

Liquid. In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.1% CHAPS, 0.09% APO-10, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human caspase-10 fused at the N- and C- termini to a His•Tag^® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000. Activity: ≥50,000 units/ml. Specific activity: ≥150,000 units/mg protein. One unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-IETD-pNA (Cat. No. 368057) per min at 25°C, pH 7.4. Purity: ≥95% by SDS-PAGE. M.W. 11,000 and 18,000 (heterodimer).

Ref.: Kang, J.J., et al. 1999. J. Biol. Chem. 274, 3189. Ng, P.W., et al. 1999. J. Biol. Chem. 274, 10301. Vincenz, C., and Dixit, V.M. 1997. J. Biol. Chem. 272, 6578. Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.