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 | -20°C |  | Ambient |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Caspase-2 Assay Kit, Colorimetric |
|  | Cat. No. 218792 |
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| Format: Cuvette or 96-well plate | | Form: 100 Tests | | Detection Method: Colorimetric | | Assay Time: 2.5-3 h | | Sample Type: Cell lysates | | Kit Contents: Cell Lysis Buffer, Reaction Buffer, VDVAD-pNA Substrate, DTT, Dilution Buffer, and a user protocol. | | Comments: This kit provides a simple and convenient assay for measuring the activity of caspase-2 and related caspases that recognize the sequence VDVAD. The assay is based on the spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the substrate VDVAD-pNA. pNA can be measured using a spectrophotometer or a microplate reader at ~405 nm. | | Ref.: Talanian, R.V., et al. 1997. J. Biol.Chem. 272, 9677. Wang, L., et al. 1994. Cell 78, 739. | | R: 22-36/38-41-52/53; S: 26-36-39-61 |
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