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  Caspase-6 Assay Kit, Colorimetric
Cat. No. 218802  
All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase

Format: Cuvette or 96-well plate
Form: 100 Tests
Detection Method: Colorimetric
Assay Time: 1.5-2.5 h
Sample Type: Cell lysate
Kit Contents: Cell Lysis Buffer, Reaction Buffer, VEID-pNA Substrate, DTT, and Dilution Buffer, and a user protocol.
Comments: This kit provides a simple and convenient assay for measuring the activity of caspase-6 and related caspases that recognize the sequence VEID. The assay is based on the spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the substrate VEID-pNA (Cat. No. 218762). pNA can be measured using a spectrophotometer or a plate reader at ~405 nm.
Ref.: Martin, L.M., et al. 1997. J. Biol. Chem. 272, 7421. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
R: 22-36/37/38-41; S: 26-36

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Related Literature:

Caspases and other Apoptosis Related Tools Brochure

Tools and Tips for Analyzing Apoptosis

Material Safety Data Sheets:
218802: Caspase-6 Assay Kit, Colorimetric - English
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All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase