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 | -20°C |  | Ambient |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Caspase-5 Assay Kit, Colorimetric |
|  | Cat. No. 218804 |
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| Format: Cuvette or 96-well plate | | Form: 100 Tests | | Detection Method: Colorimetric | | Sample Type: Cell lysate | | Kit Contents: Cell Lysis Buffer, Reaction Buffer, WEHD-pNA Substrate, DTT, Dilution Buffer, and a user protocol. | | Comments: This kit provides a simple and convenient assay for measuring the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on the spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage of the substrate WEHD-pNA. The pNA can be measured using a spectrophotometer or a plate reader at ~405 nm. | | Ref.: Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608. Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907. | | R: 22-36/37/38-41; S: 26-36 |
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