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 | -20°C |  | Ambient |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Caspase-10 Assay Kit, Fluorometric |
|  | Cat. No. 218811 |
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| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Fluorometric | | Assay Time: 2-3 h | | Sample Type: Cell lysates | | Kit Contents: Cell Lysis Buffer, Reaction Buffer, AEVD-AFC Substrate, DTT, and a user protocol. | | Comments: This kit provides a simple and convenient assay for measuring the activity of caspase-10 and related caspases that recognize the sequence AEVD. The assay is based on detection of the cleavage of the substrate. Upon cleavage of the substrate by caspase-10 or related caspases, free AFC emits a yellow-green fluorescence (~505 nm), which can be measured using a fluorometer or fluorescence microplate reader. | | Ref.: Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608. | | R: 22-36/37/38-41; S: 26-36 |
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