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Kit Resource

VEGF Signaling Pathway
  Caspase-9 Assay Kit, Colorimetric
Cat. No. 218824  
All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase

Format: Cuvette or 96-well plate
Form: 100 Tests
Detection Method: Colorimetric
Assay Time: 2-3 h
Sample Type: Cell lysate
Kit Contents: Cell Lysis Buffer, Reaction Buffer, LEHD-pNA Substrate, DTT, Dilution Buffer, and a usher protocol.
Comments: This kit provides a simple and convenient assay for measuring the activity of caspase-9 and related caspases that recognize the sequence LEHD. The assay is based on the spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the substrate LEHD-pNA. pNA can be measured using a spectrophotometer or a microplate reader at ~405 nm.
R: 22-36/37/38-44-41; S: 26-36

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Related Literature:

Caspases and other Apoptosis Related Tools Brochure

Antibodies for Cancer Research Product Guide

Tools and Tips for Analyzing Apoptosis

Material Safety Data Sheets:
218824: Caspase-9 Assay Kit, Colorimetric - English
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All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase