Liquid. In 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5. AVOID FREEZE/THAW CYCLES. Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase preferentially expressed in macrophages and microglia. Exhibits high specific activity. Specific activity: ≥30,000 mU/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit. Purity: ≥90% by SDS-PAGE. EC 3.4.22.27. Note: 1 mU = 1 milliunit. Ref.: Liuzzo, J.P., et al. 1999. Mol. Med. 5, 334. Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351. Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576. Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500. Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334. Kirschke, H., et al. 1989. Biochem. J. 264, 467. |