Lyophilized. Lyophilized from 100 mM sodium acetate, 1 mM EDTA, pH 5.5. AVOID FREEZE/THAW CYCLES. Native cathepsin S from human spleen. A major lysosomal cysteine proteinase that mediates degradation of MHC class II-invariant chain complexes in human and mouse antigen presenting cells. Cathepsin S secreted by activated macrophages has the ability to process myelin basic protein and amyloid b peptide which may be relevant to the pathogenesis of Alzheimer′s disease. It can hydrolyze elastin at both acidic and neutral pH. Specific activity: ≥50 mU/mg protein. One unit is defined as the amount of enzyme that will hydrolyze Z-VVR-AMC in 10 min at 25°C, in 100 mM KPO4, 5 mM EDTA, pH 6.5 (the assay is standardized such that 0.041 nmol AMC gives a reading of 1000 RFU. Purity: ≥90% by SDS-PAGE. Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HBC, HCV, and HIV. EC 3.4.22.27. Note: 1 mU = 1 milliunit. Ref.: Liuzzo, J.P., et al. 1999. Mol. Med. 5, 334. Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351. Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576. Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500. Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334. Kirschke, H., et al. 1989. Biochem. J. 264, 467. |
