| Format: Fluorescence microscopy | | Detection Method: Fluorescence | | Assay Time: 4-6 h | | Sample Type: Cells | | Kit Contents: Labeled Cathepsin B Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol. | | Comments: Detects cathepsin B using a cell-permeable cresyl violet labeled cathepsin B substrate, which generates red fluorescence upon cleavage. This is kit is also suitable for detecting cathepsin B activity in whole cells. | | Ref.: Boonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473. Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349. Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241. Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287. Kas J. et al. 2000. Int. J. Biol. Markers 15, 84. Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98. Frosch B.A., et al. 1999. APMIS 107, 28. Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71. Bossard M.J., 1996. J. Biol. Chem. 271, 12517. Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185. Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135. Buhling F., et al. Adv. Exp. Med. Biol. 477, 281. Leung-Toung R., et al. Med. Chem. 9, 979. | |