| Format: Fluorescence microscopy | | Detection Method: Fluorescence | | Assay Time: 4-6 h | | Sample Type: Cells | | Kit Contents: Labeled Cathepsin K Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol. | | Comments: Suitable for detection of cathepsin K in suspension or adherent cells. Detects cathepsin K using a cell-permeable cresyl violet-labeled cathepsin K substrate, which generates red fluorescence upon cleavage. | | Ref.: Boonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473. Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349. Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241. Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287. Kas J. et al. 2000. Int. J. Biol. Markers 15, 84. Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98. Frosch B.A., et al. 1999. APMIS 107, 28. Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71. Bossard M.J., 1996. J. Biol. Chem. 271, 12524. Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135. Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185. Buhling F., et al. Adv. Exp. Med. Biol. 477, 281. Leung-Toung R., et al. Med. Chem. 9, 979. | |