MOCAc-GKPILF~FRLK(Dnp)-D-R-NH2 Yellow lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates. Purity: ≥97% by HPLC. Excitation max.: ~328 nm, Emission max.: ~393 nm MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2
MOCAc = (7-Methoxycoumarin-4-yl)acetyl
Ref.: Yasuda, Y., et al. 1999. J. Biochem. 126, 260. Yasuda, Y., et al. 1999. J. Biochem. 125, 1137. |
