Lyophilized. Lyophilized from 2 mM sodium phosphate buffer, pH 6.5. AVOID FREEZE/THAW CYCLES. Native cathepsin D from human spleen. A major lysosomal aspartyl protease in mammalian cells produced as a 52 kDa proenzyme. Overexpression of cathepsin D in human breast cancers is associated with higher risk of relapse and metastasis. Cathepsin D degrades extracellular matrix and releases growth factors bound to the matrix. Specific activity: ≥300 units/mg protein. One unit is defined as the amount of enzyme that will digest acid-denatured hemoglobin in 60 min, at 37°C, pH 3.3 to release peptides soluble in TCA, as measured by an increase in absorbance of 1.0 at 280 nm. Purity: ≥98% by SDS-PAGE. Prepared from tissue of individuals that have been shown by certified test to be negative for HBsAg and for antibodies to HIV and HCV. EC 3.4.23.5, CAS 9025-26-7.

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