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| | PhosphoDetect™ Anti-Cdk1 (pThr14/pTyr15) Rabbit pAb |
|  | Cat. No. 219441 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr14 and Tyr15 phosphorylation sites of human Cdk1 | | Form: Liquid | | Formulation: In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4. | | Preservative: ≤0.1% sodium azide | | Positive Control: HeLa cells treated with nocodazole (Cat. No. 487928) | | Comments: Recognizes the ~33-35 kDa Cdk1 phosphorylated at Tyr15 and Thr14 in nocodazole-stimulated HeLa cells. | | Ref.: Borgne, A., et al. 1996. J. Biol. Chem. 271, 27847. Hunter, T. 1995. Cell 80, 225. Galaktionov, K., et al. 1995. Genes Dev. 9, 1046. Watanabe, K., et al. 1995. EMBO J. 14, 1878. Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675. McGwan, C.H. and Russel, P. 1993. EMBO J. 12, 75. |
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Detection of human Cdk1, phosphorylated on Thr14 and Tyr15, by immunoblotting. Samples: Whole cell lysate from HeLa cells stimulated with nocodazole (Cat. No. 487928) (lane 1-5). Primary antibody: PhosphoDetect™ Anti-Cdk1 (pThr14,Tyr15) Rabbit pAb (Cat. No. 219441) (1:1000) pre-incubated without peptide (lane 1), with non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), a generic phosphothreonine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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