Ac-HRYR-ACC

Lyophilized. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. A tetrapeptidyl fluorogenic substrate for human cathepsin L (k_cat = 20.8 sec^-1, K_m = 11.2 µM, and k_cat/K_m = 1.9 x 10⁶ M^-1 sec^-1). Displays ~270-fold and ~2,500-fold greater selectivities over cathepsin B (k_cat/K_m = 7.0 x 10³ M^-1 sec^-1),respectively and cathepsin K (k_cat/K_m = 7.6 x 10² M^-1 sec^-1). An excitation wavelength of 380 nm and an emission wavelength of 460 nm is recommended in order to maximize the signal of the ACC group over the background signal of uncleaved substrate. Purity: ≥95% by HPLC. ACC = 7-Amino-4-carbamoylmethylcoumarin.

Ac-His-Arg-Tyr-Arg-ACC

Ref.: Choe, Y., et al. 2006. J. Biol. Chem. 281, 12824.