3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

White crystalline powder. HYGROSCOPIC. Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON^® X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14. Purity: ≥98% by TLC. Conductivity (1.0 M, 24°C): <100 µmhos. Aggregation number 4 - 14, CMC 6 - 10 mM, micellar weight 6150. CAS 75621-03-3.

Merck Index: 14, 2044
Ref.: Chen, Y., et al. 1992. Biochemistry 31, 2415. Ofri, D., et al. 1992. J. Neurochem. 58, 628. Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823. Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA 77, 4623. Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry 31, 576. Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA 77, 6368. Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA 77, 4623.
R: 22-36/38; S: 26-36