Format: 96-well plate

Form: 96 Tests

Detection Method: Fluorometric or colorimetric

Species Reactivity: a broad range of species

Assay Time: 2.5-3 h

Sample Type: Cell extracts

Kit Contents: Human Recombinant Caspase-3, Cell Lysis Buffer, a DEVD-pNA Colorimetric Substrate, Ac-DEVD-AMC Fluorometric Substrate, Calibration Standard (p-Nitroaniline), AMC Calibration Standard, an Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol.

Comments: A convenient assay kit useful for measuring the caspase-3 and caspase-3-like activities in cell extracts using either a colorimetric or fluorometric substrate. Provided in a convenient 96-well format with all reagents necessary for preparing cell extracts, measuring caspase activity and calibrating the assay. In addition, caspase-3 is included for use as a positive control, for testing cell extracts for endogenous inhibitors, or for comparing effects of exogenous inhibitors on cellular activity versus the activity of the purified enzyme.

Ref.: Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Faleiro, L., et al. 1997. EMBO J. 16, 2271. Kothakota, S., et al. 1997. Science 278, 294. Nicholson, D.W. 1996. Nat. Biotechnol. 14, 297. Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841. Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486. Nicholson, D.W., et al. 1995. Nature 376, 37. Tewari, M., et al. 1995. Cell 81, 801. Fernandes-Alnemi, T., et al. 1994. J.Biol. Chem. 269, 30761.

R: 23/24/25-63-36/37/38-33; S: 36/37/39-45-26-23