DAG Kinase Turbid membrane suspension. In 20 mM NaCl, 10 mM potassium phosphate buffer, 1 mM DTT, 20% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant sn-1,2-diacylglycerol kinase expressed in E. coli. Membrane-bound protein that catalyzes ATP-dependent phosphorylation of 1,2-diacyl-sn-glycerol (DAG). Involved in the recycling of DAG generated during phospholipid turnover. Specific activity: ≥2 units/mg. One unit is defined as the amount of enzyme that will phosphorylate 1.0 µmol DAG per min at 25°C, pH 6.6. pH optimum 6.3 - 8.3, pI 4.0. EC 2.7.1.107. Ref.: Sanders, C.R., et al. 1996. Biochemistry 35, 8610. Van Veldhoven, P.P. 1989. Anal. Biochem. 183, 177. Preiss, J.E., et al. 1987. Methods Enzymol. 114, 294. Bell, R.M. 1986. Cell 45, 631. Preiss, J.E., et al. 1986. J. Biol. Chem. 261, 8597. Loomis, C.R., et al. 1985. J. Biol. Chem. 260, 4091. |
