Lyophilized. Salt-free. PROTECT FROM MOISTURE. Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and a2-macroglobulin. Specific activity: ≥25 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-Ala-Ala-Pro-Abu-pNA (Cat. No. 324699) per min at 25°C, pH 8.0. Contaminants: Chymotrypsin: ≥0.2%. pH optimum 7.8 - 8.5. EC 3.4.21.36, RTECS TS5052000, CAS 39445-21-1. Merck Index: 14, 3535
Ref.: Largman, C. et al. 1976. Biochemestry 15, 2491. Mandl, I. 1962. Methods in Enzymol. 5, 665. |
