White to off-white lyopilized solid. 45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content. PROTECT FROM MOISTURE. AVOID FREEZE/THAW CYCLES. A specific substrate for pancreatic elastase (km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase, Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine). Cleavage of substrate can be monitored at ~405 nm. Purity: ≥95% by HPLC. Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-a-Aminobutyric Acid) Ref.: Largman, L. 1983. Biochemistry 22, 3763. Del Mar, E.G., et al. 1980. Biochemistry 19, 468. |