Epidermal Growth Factor, Human, Recombinant, E. coli

Solid. Lyophilized from sterile-filtered PBS. Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Acts as a survival factor in preventing apoptosis. Biological activity: ED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.. Purity: ≥95% by SDS-PAGE. Contaminants: Endotoxin: <1.0 EU/mg EGF. RTECS KB4518000, CAS 62229-50-9.

Merck Index: 14, 3527
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