Epidermal Growth Factor Lyophilized. Native epidermal growth factor from mouse submaxillary glands. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Purified by size-exclusion chromatography followed by DEAE ion-exchange chromatography. Biological activity: determined by mitogenic assay with human foreskin fibroblasts. Purity: ≥98% by SDS-PAGE. Effective biological range: 5-50 ng/ml. RTECS KB4518000, CAS 62229-50-9. Merck Index: 14, 3527 Ref.: Canals, F., et al. 1992. Biochemistry 31, 4493. Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239. Sato, J., et al. 1987. Methods Enzymol. 146, 63. Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609. |