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| | PhosphoDetect™ Anti-EGFR (pTyr845) Rabbit pAb |
|  | Cat. No. 324865 |
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Anti-Epidermal Growth Factor Receptor (pTyr845) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr845 phosphorylation site of human EGFR | | Form: Liquid | | Formulation: In Dulbecco's PBS (w/o Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.4. | | Preservative: ≤0.1% sodium azide | | Positive Control: A431 cells stimulated with EGF | | Comments: Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr845 in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots. | | Ref.: Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249. Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067. Biscardi, J.S., et al. 1999. J. Biol. Chem. 274, 8335. Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139 Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227. |
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Detection of human epidermal growth factor receptor, phosphorylated on Tyr845, by immunoblotting. Samples: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr845) Rabbit pAb (Cat. No. 324865) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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