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| | PhosphoDetect™ Anti-EGFR (pTyr1068) Rabbit pAb |
|  | Cat. No. 324867 |
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Anti-Epidermal Growth Factor Receptor (pTyr1068) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1068 phosphorylation site of human EGFR | | Form: Liquid | | Formulation: In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4. | | Preservative: ≤0.1% sodium azide | | Comments: Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots. | | Ref.: Jo, M., et al. 2000. J. Biol. Chem. 275, 8806. Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572. Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249. Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067. Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139 Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227. Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192. |
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EMD Chemicals Inc. GBP list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | chicken, human, mouse, rat | IB
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Detection of human EGFR, phosphorylated at Tyr1068, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr1068) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
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