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| | PhosphoDetect™ Anti-EGFR (pTyr1148) Rabbit pAb |
|  | Cat. No. 324869 |
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Anti-Epidermal Growth Factor Receptor (pTyr1148) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1148 phosphorylation site of human EGFR | | Form: Liquid | | Formulation: In Dulbecco's PBS, 50% glycerol, 1 mg/ml BSA, pH 7.2-7.4. | | Preservative: ≤0.1% sodium azide | | Comments: Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1148. Note: 1 T = 1 test. Sufficient for 10 Western miniblots. | | Ref.: Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249. Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067. Hashimoto, A., et al. 1999. J. Biol. Chem. 274, 20139. Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227. |
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* Additional Product Information
| | chicken, human, mouse, rat | IB
See Key |
Detection of human EGFR, phosphrylated at Tyr1148, by immunoblotting. Sample: Whole cell lysate from A431 cells left unstimulated (lane 1) or stimulated with EGF (lane 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr1148) Rabbit pAb (Cat. No. 324869) (1:1000) pre-incubated without peptide (lanes 1, 2), with non phosphopetide corresponding to the immunogen (lane 3), a generic phosphotyrosine containing peptide (lane 4), or the phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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