Liquid. In 400 mM KCl, 50 mM Bis-Tris-propane, 2 mM DTT, 1 mM EDTA, 10% glycerol, pH 9.0. DO NOT VORTEX. AVOID FREEZE/THAW CYCLES. Recombinant, human estrogen receptor-b expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that shares high homology with ER-a, especially in the DNA binding protein domain, suggesting that both receptors interact with related DNA response elements. Supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells. Specific activity: ≥5000 units/mg protein. One unit is defined as the amount of estrogen receptor that will bind 1.0 pmol 3H-estradiol overnight at 4°C as measured by quantitation of estradiol:receptor complexes in a hydroxylapatite assay. Purity: ≥80% by SDS-PAGE. Aliquot in individual vials for daily use. Do not add any dilution buffers, as these may cause aggregation of the ER. Ref.: Kuiper, G.G., et al. 1997. Endocrinology 138, 863. Paech, K., et al. 1997. Science 277, 1508. Mosselman, S., et al. 1996. FEBS Lett. 392, 49. Obourn, J.D., et al. 1993. Biochemistry 32, 6229. |
