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| | PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb |
|  | Cat. No. 341292 |
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Anti-Focal Adhesion Kinase, Phospho-Specific (Tyr397) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr397 phosphorylation site of human FAK | | Form: Liquid | | Formulation: In Dulbecco's PBS (no Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Positive Control: Chicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF. | | Comments: Recognizes the ~125 kDa FAK protein when phosphorylated at Tyr397. Note: 1 T = 1 test. Sufficient for 10 Western miniblots. | | Ref.: Fornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235. Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758. Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1. |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | chicken, Drosophila, frog, human, mouse, rat | IB, IH
See Key |
Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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