Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli
rhaFGF

Solid. Lyophilized from a sterile-filtered solution of 20 mM Tris, 1 M NaCl, 50 µg BSA/µg FGF acidic, pH 7.0. Recombinant, human acidic fibroblast growth factor expressed in E. coli. Stimulates the proliferation of primary cultures of myoblasts and osteoblastic cells. Prevents myocardial apoptosis triggered by ischemia reperfusion injury. Exhibits mitogenic activity in vitro for cells or meso- and neuroectodermal origin. Its activity is greatly potentiated by addition of heparin. Biological activity: ED₅₀ = 100-300 pg/ml in the presence of 10 µg/ml heparin as monitored in a mitogenic assay by measuring the acidic FGF-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts. Purity: ≥97% by SDS-PAGE. Contaminants: endotoxin: ≤ 1 EU/µg FGF. Reconstitute to a concentration of ≥1 mg/ml with sterile PBS containing at least 0.1% HSA or BSA. Reconstituted material can be stored at +4°C for up to 3 months without detectable loss of activity. For longer storage, aliquot and freeze at -70°C. Avoid freeze/thaw cycles of solutions. RTECS LK3641300, CAS 106096-92-8.

Merck Index: 14, 4072
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