Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
rhbFGF

Lyophilized. Lyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0. Recombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells. Biological activity: ED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxin: ≤1 EU/µg FGF. RTECS LK3641325, CAS 106096-93-9.

Merck Index: 14, 4072
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Sold under license of U.S. Patent 5,155,214.